The FastGene ® PAGE Protein System (PG01) can easiliy be upgraded for Western Blotting by ordering the FastGene ® Western Blot Components (PG09, see below). The FastGene ® Western Blot System (PG08) uses the same FastGene ® chamber tank (PG05) and FastGene ® Gel/Blot chamber lid as the FastGene ® PAGE Protein System (PG01). For more detailed information, please have a look at the manual (click here).įigure: General setup of the FastGene ® Western Blot System.Ĭontent and accessories Content of FastGene ® Western Blot System (PG08) Content Each Vial contains 50ug total protein per. Gel and membrane can be easily sandwiched in the blot connector, which is assembled via an easy-to-use lock mechanism.īelow you find an image of the general Western Blot setup. Pre-mixed molecular weight markers with 6 over-expressed cell lysates with Myc/DDK tags covering 28 to 158 KDa. Precision Plus Protein WesternC Standards are combination protein standards containing ten prestained, Strep-tagged recombinant proteins, enabling both fluorescent visualization on gels and colorimetric or chemiluminescent western blot detection with StrepTactin-AP or StrepTactin-HRP Conjugate. The cool pack absobs generated running heat, avoiding the possibility of power loss. The system includes a cool pack, which is installed during the run. The Western Blot Systems contains all the necessary components for reliable protein transfer and comes with detailed guidelines to successfully perform transfer and Western Blot analysis. This product is designed for use as molecular weight markers in Western blots. Find a range of innovative, high-quality Thermo Scientific Pierce products, including the Pierce antibodies, SuperSignal chemiluminescent substrates, Pierce BCA Protein Assays and other protein biology research tools and reagents. protein was derived from a calibration curve using the biotinylated markers. After transferring the protein bands onto the blot paper, stain the paper with Ponceau-S. The FastGene ® Western Blot System is an efficient device to perform the first Western Blot protein transfer step from gel to membrane via a wet electropohoretic transfer technique. Contact our technical support scientists for experienced troubleshooting and product advice: 5345. If you want to do with unstained ladder yet, I recommend to do all steps of western blot just routinely do. Some pre-stained protein ladders fluoresce strongly and can interfere with detection of proteins expressed at a low level. The firm binding of the proteins to the membrane allows a subsequent protein detection by choosing from a variety of staining or immunological methods. Protein bands that were separated by size after PAGE are transferred and immobilized on a carrier membrane. Details Biochemical protein analysis via blottingīlotting of proteins is a powerful biochemical method for the detection of proteins.
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